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Chlorhexidine gel associated with papain in pulp tissue dissolution.

Gabriel Couto De Oliveira¹, Caio Souza Ferraz, Carlos Vieira Andrade Júnior

¹Southwest Bahia State University-UESB, Jequié, Bahia, Brazil.

Restorative Dentistry & Endodontics

|December 5, 2013

Summary

This study compared 2% chlorhexidine and 8% papain gels against 5.25% sodium hypochlorite for dissolving bovine pulp tissue. Sodium hypochlorite demonstrated superior dissolution capacity compared to papain-based agents.

Keywords:

Chlorhexidine Endodontics Papain

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Area of Science:

Biomaterials Science
Dental Materials Research
Tissue Dissolution Studies

Background:

Effective dissolution of pulp tissue is crucial in endodontic procedures.
Sodium hypochlorite is a widely used irrigant but can have limitations.
Alternative agents are being investigated for pulp tissue dissolution.

Purpose of the Study:

To compare the efficacy of 2% chlorhexidine gel combined with 8% papain gel against 5.25% sodium hypochlorite in dissolving bovine pulp tissue.
To evaluate the dissolution capacity of papain and chlorhexidine formulations.
To assess the influence of chlorhexidine on papain's tissue dissolution potential.

Main Methods:

Ninety bovine pulps were fragmented into 5mm sections.
Fragments were divided into six groups: 8% papain, 2% chlorhexidine, 2% chlorhexidine + 8% papain, 0.9% saline, 2.5% sodium hypochlorite, and 5.25% sodium hypochlorite.
Pulp fragments were incubated for 30, 60, 90, and 120 minutes to measure dissolution.

Main Results:

5.25% sodium hypochlorite showed significantly greater pulp dissolution than pure papain.
Chlorhexidine combined with papain promoted more dissolution than saline or 2% chlorhexidine alone.
After 120 minutes, 5.25% and 2.5% sodium hypochlorite achieved 100% dissolution, papain achieved 61%, chlorhexidine + papain achieved 55%, and chlorhexidine alone achieved 3%.

Conclusions:

8% papain gel, alone or with chlorhexidine, can dissolve bovine pulp tissue.
The dissolution capacity of papain-based agents was less than that of 5.25% sodium hypochlorite.
Further research may explore optimizing papain and chlorhexidine formulations for endodontic applications.