Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Operons02:09

Operons

44.0K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
44.0K
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

94
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
94
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

3.1K
The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular Switch Controlling Expression of the Mannose-Specific Adhesin, Msa, in <i>Lactobacillus plantarum</i>.

Applied and environmental microbiology·2019
Same author

Compliance with Guidelines on Thromboprophylaxis for Acutely Admitted Medical Patients.

Advances in therapy·2018
Same author

Expression, Purification and Characterization of GMZ2'.10C, a Complex Disulphide-Bonded Fusion Protein Vaccine Candidate against the Asexual and Sexual Life-Stages of the Malaria-Causing Plasmodium falciparum Parasite.

Pharmaceutical research·2017
Same author

Recombinant expression of Laceyella sacchari thermitase in Lactococcus lactis.

Protein expression and purification·2013
Same author

Anchorless surface associated glycolytic enzymes from Lactobacillus plantarum 299v bind to epithelial cells and extracellular matrix proteins.

Microbiological research·2013
Same author

The capillary dysfunction hypothesis of Alzheimer's disease.

Neurobiology of aging·2012

Related Experiment Video

Updated: May 5, 2026

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

16.9K

Recombinant protein expression in Lactococcus lactis using the P170 expression system.

Casper M Jørgensen1, Astrid Vrang, Søren M Madsen

  • 1Bioneer A/S, Bacterial Expression Group, Hørsholm, Denmark.

FEMS Microbiology Letters
|December 6, 2013
PubMed
Summary
This summary is machine-generated.

Lactococcus lactis is a safe host for producing recombinant proteins. Combining the P170 expression system with REED™ technology boosts Staphylococcus aureus nuclease production to 2.5 g L(-1).

Keywords:
REED™ technologyfermentationoptimised production strainssignal peptides

More Related Videos

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
09:46

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

Published on: February 25, 2010

46.2K
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

20.8K

Related Experiment Videos

Last Updated: May 5, 2026

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

16.9K
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
09:46

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

Published on: February 25, 2010

46.2K
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

20.8K

Area of Science:

  • Microbiology
  • Biotechnology
  • Molecular Biology

Background:

  • Lactococcus lactis offers advantages for recombinant protein production due to its GRAS status and endotoxin-free nature.
  • Optimized L. lactis strains secrete stable proteins into the medium, simplifying downstream processing.
  • The P170 inducible promoter system, regulated by lactate accumulation, is suitable for controlled protein expression.

Purpose of the Study:

  • To optimize the P170 expression system in L. lactis for enhanced recombinant protein yield.
  • To overcome lactate-induced growth inhibition in L. lactis fermentation processes.
  • To achieve high-level production of Staphylococcus aureus nuclease using a combined expression and purification strategy.

Main Methods:

  • Optimization of promoter strength, signal peptides, and selection of high-productivity L. lactis strains.
  • Development of a fermentation process using animal-component-free media and minimal process control.
  • Integration of the P170 system with REED™ technology for real-time lactate removal via electro-dialysis.

Main Results:

  • Significant improvements in promoter strength and strain productivity were achieved.
  • Recombinant proteins were successfully produced in a simple batch fermentation process.
  • The combined P170 and REED™ system enabled Staphylococcus aureus nuclease production of 2.5 g L(-1), overcoming previous yield limitations.

Conclusions:

  • The optimized P170 expression system in L. lactis is highly effective for recombinant protein production.
  • REED™ technology successfully mitigates lactate inhibition, enabling high-yield fermentations.
  • This integrated approach provides a robust and scalable platform for industrial-scale biopharmaceutical manufacturing.