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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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A practical guide to sequencing genomes and transcriptomes.

Alejandro Sanchez-Flores, Cei Abreu-Goodger1

  • 1Unidad Universitaria de Secuenciacion Masiva de DNA, Universidad Nacional Autonoma de Mexico, Cuernavaca, Mor. Mexico. alexsf@ibt.unam.mx.

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Summary
This summary is machine-generated.

This review details second and third generation DNA sequencing technologies, offering a practical cost-benefit analysis and project map. It guides scientists in choosing optimal sequencing strategies for genomic and transcriptomic projects.

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Area of Science:

  • Genomics and transcriptomics
  • Bioinformatics
  • Molecular biology

Background:

  • Advancements in DNA sequencing technologies have led to exponential growth in genomic and transcriptomic data.
  • These technologies offer significant advantages but also present challenges that require careful consideration for each new method.

Purpose of the Study:

  • To review second and third generation DNA sequencing technologies.
  • To analyze their impact on genome and transcriptome studies.
  • To provide a practical framework for selecting sequencing project strategies.

Main Methods:

  • Review of second and third generation DNA sequencing technologies.
  • Development of a "sequencing project map" with cost-benefit analysis.
  • Classification of workflows based on project goals and experimental design.

Main Results:

  • Identification of key factors for successful sequencing projects.
  • Presentation of a graphical cost-benefit analysis for genome and transcriptome projects.
  • Comparison of pros and cons for widely adopted experimental designs and sequencing technologies.

Conclusions:

  • The review provides a valuable resource for scientists planning sequencing projects.
  • It aids in selecting the most appropriate sequencing strategy based on project-specific needs.
  • The "sequencing project map" facilitates informed decision-making in genomics and transcriptomics research.