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Updated: May 5, 2026
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Published on: January 28, 2021
Apolipoprotein A-V level may contribute to the development of obesity-associated dyslipidemia
Yayoi Yoshino1, Tomoo Okada, Yuriko Abe
1Department of Pediatrics and Child Health, Nihon University School of Medicine 30-1, Oyaguchi Kamicho, Itabashi-ku, Tokyo 173-8610, Japan.
Insights
In obese children with hyperinsulinemia, lower apolipoprotein A-V (apoA-V) levels are linked to higher triglycerides and lower HDL cholesterol. Insulin influences apoA-V, contributing to dyslipidemia in obesity.
Area of Science:
- Endocrinology
- Metabolic Syndrome
- Lipid Metabolism
Background:
- Insulin suppresses apolipoprotein A-V (apoA-V) gene expression in mice.
- Dyslipidemia is common in obese children with hyperinsulinemia.
Purpose of the Study:
- Investigate the association between apoA-V levels and dyslipidemias in obese children with hyperinsulinemia.
Main Methods:
- Studied 17 obese children (11.8 ± 2.4 years).
- Measured total cholesterol, HDL cholesterol, triglycerides, apoA-V, and insulin levels.
Main Results:
- Obese children with hyperinsulinemia had higher triglycerides, lower HDL cholesterol, and lower apoA-V levels.
- ApoA-V correlated negatively with triglycerides and insulin, and positively with HDL cholesterol.
- Insulin determined triglyceride levels, while apoA-V determined HDL cholesterol levels independently.
Conclusions:
- Insulin may regulate serum apoA-V levels in obese individuals.
- ApoA-V may contribute to obesity-associated dyslipidemia.
Aim:
In a mice study, insulin suppressed apolipoprotein A-V (apoA-V) gene expression in a dose dependent manner. Thus, we investigated the association between apoA-V levels and dyslipidemias in obese children with hyperinsulinemia.
Methods:
The subjects were 17 obese children (15 male, 2 female) aged 11.8 ± 2.4 years. Total cholesterol (TC), high-density lipoprotein cholesterol (HDLC), triglyceride (TG), apoA-V and insulin levels were determined.
Results:
Obese children with hyperinsulinemia had greater percent overweight, higher TG level, lower HDLC level and lower apoA-V level than those without hyperinsulinemia. In simple regression analysis, apoA-V level correlated negatively with TG (r = -0.613, p = 0.0152) and insulin levels (r = -0.566, p = 0.0279), and positively correlated with HDLC (r = 0.811, p = 0.0002). In stepwise regression analysis, insulin level emerged as the independent determinant of TG level after apoA-V level was taken into account, whereas apoA-V emerged as the independent determinant of HDLC level after adjusting for insulin level.
Conclusions:
Insulin may be a potent regulator of serum apoA-V level in obesity, and apoA-V level may partly contribute to the development of obesity-associated dyslipidemia.
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