Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

3.8K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
3.8K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K
Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

3.0K
Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
3.0K
Disorders of Leukocytes01:27

Disorders of Leukocytes

2.3K
Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune...
2.3K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.1K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resolution of PML after Treatment with Virus-Specific T Cells and HCT.

The New England journal of medicine·2026
Same author

Impact of blinatumomab and inotuzumab exposure on apheresis composition for CAR T in patients with B-cell acute lymphoblastic leukemia.

Cytotherapy·2026
Same author

Intra-apheresis CD34+ cell count: A dynamic approach to predicting peripheral blood stem cell collection yield.

Transfusion·2026
Same author

Complete Response in Hairy Cell Leukemia to Anti-CD22 CAR T-Cell Therapy.

JCO precision oncology·2025
Same author

Topical TLR7 agonist and radiotherapy in patients with metastatic breast cancer.

Journal for immunotherapy of cancer·2025
Same author

A novel approach for the co-delivery of 5-fluorouracil and everolimus for breast cancer combination therapy: stimuli-responsive chitosan hydrogel embedded with mesoporous silica nanoparticles.

Journal of translational medicine·2025

Related Experiment Video

Updated: May 5, 2026

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

2.0K

Leukemia cells induce changes in human bone marrow stromal cells.

Sara Civini, Ping Jin, Jiaqiang Ren

  • 1Cell Processing Section, Department of Transfusion Medicine, Clinical Center, National Institutes of Health (NIH), Building 10, Room 3C720, 9000 Rockville Pike, Bethesda, MD 20892-1184, USA. DStroncek@cc.nih.gov.

Journal of Translational Medicine
|December 6, 2013
PubMed
Summary
This summary is machine-generated.

Bone marrow stromal cells (BMSCs) interact with leukemia cells, altering their gene expression and cytokine secretion. This interaction creates a niche favoring leukemia stem cell growth.

More Related Videos

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
10:03

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Published on: August 1, 2017

11.8K
Modeling Chemotherapy Resistant Leukemia In Vitro
08:41

Modeling Chemotherapy Resistant Leukemia In Vitro

Published on: February 9, 2016

8.6K

Related Experiment Videos

Last Updated: May 5, 2026

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

2.0K
Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
10:03

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Published on: August 1, 2017

11.8K
Modeling Chemotherapy Resistant Leukemia In Vitro
08:41

Modeling Chemotherapy Resistant Leukemia In Vitro

Published on: February 9, 2016

8.6K

Area of Science:

  • Hematology
  • Cancer Biology
  • Cell Biology

Background:

  • Bone marrow stromal cells (BMSCs) are crucial multipotent cells within the hematopoietic niche, supporting normal hematopoiesis but also nurturing leukemia, tumors, and metastasis.
  • BMSCs secrete factors that contribute to a pro-tumorigenic bone marrow microenvironment, and their secretome is influenced by inflammatory cytokines like IFN-γ and TNF-α.

Purpose of the Study:

  • To investigate how leukemia cells influence the secretome of BMSCs.
  • To understand the molecular interactions between leukemia cells and BMSCs in the bone marrow niche.

Main Methods:

  • Co-culture of human BMSCs with myeloid leukemia cell lines (TF-1, TF-1α, K562) using a trans-well system.
  • Global gene expression analysis of co-cultured cells and protein analysis of culture supernatants.
  • Control co-culture experiments using CD34+ cells and BMSCs.

Main Results:

  • Leukemia cell co-culture altered gene expression in BMSCs, up-regulating pro-inflammatory genes, including IL-17 signaling, IL-8, and CCL2.
  • Leukemia cells themselves showed changes in gene expression related to stem cell pluripotency, TGF-β, and carcinoma signaling pathways.
  • In contrast, co-culture with CD34+ cells led to up-regulation of purine metabolism, mTOR, and EIF2 signaling pathways in BMSCs.

Conclusions:

  • BMSCs exhibit altered cytokine and chemokine secretion profiles in response to leukemia cells.
  • The interaction between BMSCs and leukemia cells promotes a niche that favors leukemia stem cells.