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Updated: May 5, 2026

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato
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Virus-induced gene complementation in tomato.

Jinhua Kong1, Weiwei Chen1, Jiajia Shen1

  • 1Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.

Plant Signaling & Behavior
|December 6, 2013
PubMed
Summary
This summary is machine-generated.

Virus-induced gene complementation (VIGC) uses a plant virus for transient gene overexpression, enabling tomato mutants to ripen. This efficient method aids in understanding gene function without stable transformation.

Keywords:
Potato virus XVIGCcolourless non-ripeningfunctional complementationplant virus technologyripening inhibitor

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Area of Science:

  • Plant biology
  • Molecular genetics
  • Biotechnology

Background:

  • Tomato ripening is a complex process regulated by intricate genetic networks.
  • Understanding gene function in plants often requires stable genetic modification, which can be time-consuming.
  • Transient gene expression systems offer a faster alternative for functional genomics.

Purpose of the Study:

  • To introduce and validate Virus-Induced Gene Complementation (VIGC) as a novel method for plant gene functional analysis.
  • To investigate the role of specific genes in tomato fruit ripening using VIGC.
  • To explore the potential of VIGC for rapid "gain-of-function" studies in plants.

Main Methods:

  • Virus-Induced Gene Complementation (VIGC) utilizing Potato virus X was employed for transient gene overexpression.
  • Tomato mutants exhibiting non-ripening phenotypes were subjected to VIGC.
  • The study focused on complementing endogenous genes to restore the ripening process.

Main Results:

  • VIGC successfully complemented tomato mutants, restoring the ability of fruits to ripen.
  • This transient overexpression approach demonstrated a "gain-of-function" effect without stable transformation.
  • The study identified a potential fruit-specific transcriptional network involving key transcription factors in tomato ripening.

Conclusions:

  • Virus-Induced Gene Complementation (VIGC) is a novel, efficient, and feasible strategy for plant gene functional analysis.
  • VIGC offers a rapid "gain-of-function" approach, bypassing the need for stable transformation.
  • The technology facilitates the discovery of gene networks regulating important plant traits like fruit ripening.