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Updated: May 5, 2026

Dissection and Flat-mounting of the Threespine Stickleback Branchial Skeleton
Published on: May 7, 2016
Larval anatomy of the pterobranch Cephalodiscus gracilis supports secondarily derived sessility concordant with
1Humboldt-Universität zu Berlin, Comparative Zoology, Berlin, Germany, tstach@zoosyst-berlin.de.
Abstract:
Pterobranchs have been interpreted as "missing links" combining primitive invertebrate features with advanced vertebrate-like characteristics. The first detailed morphological description of an ontogenetic stage of a pterobranch, based on digital 3D-reconstruction at electron microscopic resolution, reveals a triploblastic animal with monociliated epithelia, an extensive coelomic cavity, a through gut with an asymmetrically developed gill slit but no signs of planktonic specializations, such as ciliated bands. Therefore, this crawling larva supports the hypothesis proposed in previous molecular phylogenetic studies that pterobranchs could be derived within enteropneusts rather than being "missing links".
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