Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in
T Ohtani1, S Kiyokawa, T Ohgawara
1Institute of Biological Sciences, University of Tsukuba, Sakura-mura, 305, Ibaraki, Japan.
Abstract:
The nucleotide sequence of a tobacco (Nicotiana tabacum) chromosomal DNA segment(t3-ars) capable of replication in yeast (ars: autonomously replicating sequences) is presented. The subcloned region (618 bp) contained 11 bp consensus (5' A/TTTTATPuTTTA/T 3') essential for several yeast ars, and 73% A and T. Unique 70 bp repetitive sequences resided next to this sequence. Thirty-two bp AT repeats were also seen in the neighbourhood of the repetitive sequence. The hybrid plasmid containing t3-ars was mitotically stabilized by the help of yeast centromere (CEN4).
More Related Videos
08:40Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
Published on: October 21, 2022
05:22Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
Published on: September 13, 2024
Related Concept Videos
Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
RNA Stability
RNA Stability
Non-nuclear Inheritance
DNA as a Genetic Template
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
