High-throughput identification of chemical endomembrane cycling disruptors utilizing tobacco pollen

Endomembrane cycling processes in plants remain mostly intractable through classical genetic interrogation. Chemical disruption of these processes provides an opportunity to slow or inhibit these processes for study. Tobacco pollen, which is dependent upon endomembrane cycling for tube growth, provides a plant system that is amenable to high-throughput screening of chemical disruptors. We describe here the process that allowed the identification of over 360 endomembrane cycling disruptors.