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Updated: Aug 9, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Analysis of picogram quantities of indole-3-acetic acid by high performance liquid chromatography-fluorescence
A Crozier1, K Loferski, J B Zaerr
1Department of Forest Science, Oregon State University, 97331, Corvallis, OR, USA.
Abstract:
The use of spectrofluorimeter coupled to a reverse phase high performance liquid chromatography column permits selective detection of indole-3-acetic acid at the low picogram level. The value of the technique is demonstrated by the analysis of endogenous IAA in elongating shoots, xylem sap and callus of Douglas-fir. The data are also used to illustrate a procedure whereby the accuracy of chromatographic analyses can be verified within definable probability limits.
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