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Related Experiment Videos

A replication origin is turned off by an origin-"silencer" sequence.

I Patel, D Bastia

    Cell
    |December 5, 1986
    PubMed
    Summary
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    A DNA silencer near the R6K origin gamma prevents its use by initiating RNA. Removing this silencer activates the origin, demonstrating its role in replication control.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Replication Origins

    Background:

    • The R6K plasmid chromosome possesses multiple replication origins.
    • Origin gamma is often unused and remains dormant in some miniplasmid variants.

    Purpose of the Study:

    • To investigate the mechanism behind the inactivation of the R6K origin gamma.
    • To identify and characterize the function of the origin silencer sequence.

    Main Methods:

    • Investigating the function of a natural silencer adjacent to the minimal ori gamma sequence.
    • Analyzing the silencer's cis-acting nature, polarity, and orientation dependence.
    • Examining the proposed RNA initiation mechanism and its effect on origin function.

    Main Results:

    Related Experiment Videos

    • A natural silencer located downstream of ori gamma inactivates the origin.
    • The silencer functions in cis, exhibits polarity, and requires a specific orientation.
    • Silencer removal prevents silencer RNA synthesis and reactivates the origin.

    Conclusions:

    • The origin silencer inhibits R6K origin gamma activity through RNA initiation.
    • The silencer competes with primer RNA or disrupts origin structure.
    • Deletion of the silencer derepresses origin gamma, confirming its regulatory role.