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Related Experiment Video

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Analysis of Cripto expression during mouse cardiac myocyte differentiation.

Jiu-Zhen Jin1, Min Tan, Jixiang Ding

  • 1Department of Molecular, Cellular and Craniofacial Biology and Birth Defects Center, University of Louisville, Louisville, KY, USA.

The International Journal of Developmental Biology
|December 6, 2013
PubMed
Summary

The mouse Cripto gene is transiently expressed in early cardiac progenitor cells. Cripto-expressing cells contribute to the left ventricle, right ventricle, and outflow tract myocardium during development.

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Area of Science:

  • Developmental biology
  • Cardiovascular research
  • Gene expression studies

Background:

  • Vertebrate cardiac progenitor cells originate from the first and second heart fields.
  • First heart field cells form left ventricle and atria myocardium.
  • Second heart field cells contribute to outflow tract, right ventricle, and atria.

Purpose of the Study:

  • To investigate the expression pattern of the mouse Cripto gene.
  • To determine the lineage of Cripto-expressing cells.
  • To understand the relationship between Cripto and cardiac myocyte differentiation.

Main Methods:

  • Utilized a Cripto-LacZ allele in mice.
  • Tracked Cripto-expressing cell progeny during heart development.
  • Analyzed gene expression at specific embryonic stages (late head fold to E8.5).

Main Results:

  • Mouse Cripto gene is expressed in the first heart field (cardiac crescent) at late head fold stages.
  • Cripto expression is later detected in the outflow tract by embryonic day 8.5.
  • Cripto-expressing progeny contribute to the myocardium of the entire outflow tract, right ventricle, and a majority of the left ventricle.

Conclusions:

  • Cripto is transiently expressed in differentiating myocardial cells of the left ventricle, right ventricle, and outflow tract.
  • Cripto expression is downregulated as these cardiac cells further differentiate.
  • Cripto plays a role in the development of specific regions of the embryonic heart.