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DPPrimer - A Degenerate PCR Primer Design Tool

Shachi Gahoi¹, L Arya, Rai Anil

¹National Bureau of Plant Genetic Resources, Pusa campus, New Delhi-12.

|December 6, 2013

Abstract

Unlabelled:

Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of this tool include platform independence and user friendliness in primer design. Embedded features such as search for functional domains, similarity score selection and phylogebetic tree further enhance the user friendliness of DPPrimer tool. Performance of DPPrimer tool was evaluated by successful PCR amplification of ADP-glucose phosphorylase genes from wheat, barley and rice.

Availability:

DPPrimer is freely accessible at http://202.141.12.147/DGEN_tool/index.html.

Keywords:

Degeneracy PCR Primer design

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