Na fluxes in human mononuclear leucocytes

Unidirectional 22Na fluxes were investigated in human peripheral mononuclear leucocytes. Ouabain inhibited about 60% of Na efflux and the addition of bumetanide further reduced Na efflux rate by about 45%, suggesting the presence of a transport pathway capable of extruding Na against its gradient. Prostaglandins E1 and E2 and exogenous cAMP were found to be potent inhibitors of the bumetanide-sensitive Na efflux without affecting the ouabain-sensitive or the ouabain and bumetanide resistant Na effluxes.