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Updated: May 5, 2026
Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
The establishment of Saccharomyces boulardii surface display system using a single expression vector
Tiantian Wang1, Hui Sun1, Jie Zhang2
1Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China.
Researchers developed a novel Saccharomyces boulardii surface display system for heterologous protein expression. This breakthrough enables potential live vaccines against eukaryotic pathogens using this probiotic yeast.
Area of Science:
- Microbiology
- Biotechnology
- Molecular Biology
Background:
- Saccharomyces boulardii is a probiotic yeast with potential applications in biotechnology.
- Surface display systems are valuable tools for protein presentation and vaccine development.
- Existing systems often lack efficiency or are not suitable for probiotic organisms.
Purpose of the Study:
- To establish a robust surface display system on Saccharomyces boulardii.
- To demonstrate the display of heterologous proteins on the yeast cell surface.
- To explore the potential of this system for developing live vaccines.
Main Methods:
- Construction of a single expression vector (pSDSb) based on a known co-expression system.
- Utilizing the AGA1 gene from S. boulardii as a cell wall anchor.
- Confirmation of heterologous protein display using enhanced green fluorescent protein (EGFP) and EtMic2 proteins.
Main Results:
- Successfully established a Saccharomyces boulardii surface display system using a single vector.
- Confirmed the display of EGFP and chicken Eimeria tenella Microneme-2 proteins on the yeast surface.
- This marks the first instance of heterologous protein display on S. boulardii.
Conclusions:
- The developed S. boulardii surface display system is effective for presenting heterologous proteins.
- This system holds significant promise for the development of live vaccines, particularly against eukaryotic pathogens.
- The probiotic nature of S. boulardii enhances the potential value of this surface display technology.
