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Updated: May 5, 2026

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy
Published on: April 9, 2017
Fusicoccin receptors. Evidence for endogenous ligand
P Aducci1, G Crosetti, R Federico
1Istituto di Chimica Biologica dell'Università, Città Universitaria, I-00185, Rome, Italy.
Abstract:
The binding of fusicoccin to the microsomal preparations of maize roots in vitro is increased several-fold when segments of the tissue are washed for 2 h in distilled water before homogenization. Addition of freeze-dried wash solution to microsomal preparations of spinach leaves or fresh roots, washed roots, or coleoptiles of maize inhibited the binding of fusicoccin to particulate fractions. The freeze-dried material also blocked fusicoccin-promoted H(+) extrusion from maize root segments. Roots may contain one or more water-soluble compounds competing with fusicoccin at the receptor level; such ligands might play a physiological role as modulators of the H(+)/K(+) exchange system in higher plants.
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