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Updated: May 5, 2026
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
Published on: November 10, 2016
Studies on ethylene binding by cell-free preparations from cotyledons of Phaseolus vulgaris L. : I. Separation and
T Bengochea1, J H Dodds, D E Evans
1Department of Botany and Microbiology, Unversity College of Wales, SY23 3DA, Aberystwyth, Dyfed, UK.
Abstract:
The preparation is described of a cell-free system from developing cotyledons of Phaseolus vulgaris cv. Canadian Wonder which is capable of binding ethylene. The binding is saturable and the apparent dissociation constant for ethylene is 6.4·10(-10) M in solution. The binding site is associated with subcellular particles and treatment with Triton X-100 results in substantial solubilisation of the activity. The kinetics of association and dissociation of the ligand and the binding site are described. The system is heat labile and binding activity is diminished by treatment with some proteolytic enzymes.
