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Related Concept Videos

RNA Stability01:53

RNA Stability

31.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Translational Regulation01:29

Translational Regulation

877
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
877
Improving Translational Accuracy02:07

Improving Translational Accuracy

11.6K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
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Related Experiment Video

Updated: May 5, 2026

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
06:31

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

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Ribosomal RNA integrity and rate of seed germination.

P A Brocklehurst1, R S Fraser

  • 1National Vegetable Research Station, CV35 9EF, Wellesbourne, Warwick, U.K..

Planta
|December 7, 2013
PubMed
Summary

Seed germination success is linked to ribosomal RNA (rRNA) integrity. Dry seeds with intact rRNA showed better germination rates, with rRNA degrading upon seed hydration.

Area of Science:

  • Plant biology
  • Molecular biology
  • Seed physiology

Background:

  • Ribosomal RNA (rRNA) is crucial for protein synthesis and cell viability.
  • Seed quality and germination are influenced by molecular integrity.
  • Understanding rRNA stability in seeds can provide insights into germination potential.

Purpose of the Study:

  • To investigate the relationship between ribosomal RNA (rRNA) integrity and seed germination rates.
  • To assess rRNA degradation during the initial stages of seed imbibition.

Main Methods:

  • Analysis of ribosomal RNA (rRNA) integrity using denaturing gel electrophoresis.
  • Testing rRNA integrity in carrot and Nicotiana seeds stored for different durations and harvested at varying maturity stages.

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A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
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Last Updated: May 5, 2026

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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Main Results:

  • A positive correlation was observed between the integrity of dry seed rRNA and subsequent germination rate within both carrot and Nicotiana species.
  • Carrot seeds exhibited significant rRNA degradation in both embryo and endosperm within two days of imbibition.

Conclusions:

  • Ribosomal RNA integrity in dry seeds serves as a reliable indicator of germination potential.
  • The process of seed imbibition triggers rapid rRNA degradation, impacting cellular function.