Related Experiment Video
Updated: May 5, 2026

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes
Published on: December 5, 2025
Localization of the replicase recognition site within brome mosaic virus RNA by hybrid-arrested RNA synthesis
P Ahlquist1, J J Bujarski, P Kaesberg
1Biophysics Laboratory of the Graduate School, University of Wisconsin-Madison, 53706, Madison, WI, U.S.A..
Abstract:
3' terminal fragments of BMV RNA as short as 153 bases in length serve as efficient templates in vitro for BMV-specific RNA polymerase. Template activity of such fragments or of native BMV RNA is abolished when cDNA fragments as short as 39 bases are hybridized to their 3' termini. Hybridization of cDNa fragments to regions of BMV RNA 200 or more bases distal to the 3' end has no discernible effect on initiation and little effect on elongation. We conclude that BMV RNA polymerase initiates binding with an RNA template through a mechanism mediated by the tRNA-like 3' end of BMV RNA, requiring at least some of the last 39, but no more than the last 153 bases.
Related Concept Videos
Viruses with RNA Genomes
Leaky Scanning
In-situ Hybridization
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
siRNA - Small Interfering RNAs
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

