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Updated: May 5, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
Characterization of developing oat seed mRNA: evidence for many globulin mRNAs
S Fabijanski1, G J Matlashewski, I Altosaar
1Department of Biochemistry, School of Medicine, University of Ottawa, K1N9B4, Ottawa, Ontario, Canada.
Abstract:
Polyadenylated mRNA from developing oat (Avena sativa L.) seeds was isolated and analyzed. Prominent mRNA species of 18S, 15S and 12S were observed; the 18S mRNA was judged to be esentially free of ribosomal RNA by hybridization analysis. Size fractionation andin vitro translation of this mRNA was performed. SDS, IEF-SDS gel electrophoresis and immunoprecipitation were used to analyze the translation products. It is shown that globulin mRNA (18S) accounts for roughly 30% of the total mRNA in developing seeds, the 12S and 15S mRNAs accounting for the remainder. The 18S mRNA directs the synthesis of a series of distinct but related polypeptides, suggesting that some of the heterogeneity seen in the oat globulins is at the amino acid sequence level.
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