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Updated: May 5, 2026

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
Published on: July 29, 2019
Sequence of histidyl tRNA, present as a chloroplast insert in mtDNA ofZea mays
K P Iams1, J E Heckman, J H Sinclair
1ILRAD, P.O. Box 30709, Nairobi, Kenya.
Abstract:
Unfractionated tRNA, isolated from maize mitochondria, has been specifically labeled at the -CCA end and used to recover a tRNA gene-bearing fragment from a clone bank of maize mitochondrial DNA. This gene has been mapped, sequenced and found to carry the anticodon for histidine. The sequence of the gene and that of bases in its near vicinity are identical to maize chloroplast tRNA(His), although sequences more distant on the fragment are not homologous with cpDNA. The junction of the cpDNA insert has been sequenced.
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