Far-red mediated polyribosome formation in radish cotyledons : Effect of endogenous ribonucleases on polyribosome
P Fourcroy1, C Lambert, P Rollin
1Centre de Recherche de Biochimie et Physiologie Cellulaires, Laboratoire de Photobiologie (LA 203), Faculté des Sciences de Rouen, F-76130, Mont-Saint-Aignan, France.
Abstract:
The total ribosome content of radish cotyledons increases during the first 2-3 days of germination both in darkness and under far-red light irradiation; ribonuclease activity is not under phytochrome control during this period. Changes in ribonuclease activity interfere with the analysis of the polyribosomal population. A maximal ratio of polysomes to monosomes is observed 12 h after the onset of far-red light and then it decreases. A 12 h far-red irradiation stimulates the in vivo incorporation of amino acids into proteins. This stimulation persists when seedlings are transferred for 4 h to the dark.
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