Related Experiment Video
Updated: Aug 1, 2026
07:47
Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
Published on: January 1, 2016
11.3K
Multistage disruption of protoplasts by dikegulac
Planta
|December 7, 2013
Summary
Dikegulac, a plant growth regulator, inhibits division and damages cells in isolated tobacco protoplasts. It affects metabolic functions in dividing cells, impacting cellular structures like the plasmalemma and tonoplast.
Area of Science:
- Plant Physiology
- Cell Biology
Background:
- Dikegulac (2,3:4,6 di-o-isopropylidine-2-keto-I-gulonate) is a known plant growth regulator.
- It selectively inhibits metabolic functions in dividing cells versus stationary cells in suspension cultures.
Purpose of the Study:
- To investigate the effects of dikegulac on isolated tobacco protoplasts.
- To determine if dikegulac exhibits analogous effects on protoplasts as observed in suspension cultures.
Main Methods:
- Treatment of isolated tobacco protoplasts with varying concentrations of dikegulac.
- Microscopic observation of protoplast division, cytoplasmic damage, and organelle integrity.
Main Results:
- Low concentrations of dikegulac partially suppressed protoplast division.
- Higher concentrations induced visible cytoplasmic damage, likely initiating at the plasmalemma.
- Vacuoles remained intact initially, with later tonoplast disruption observed.
Conclusions:
- Dikegulac affects isolated tobacco protoplasts similarly to suspension cultures.
- The compound inhibits cell division and causes progressive cellular damage in protoplasts.
- The observed effects suggest dikegulac targets fundamental metabolic processes in dividing plant cells.
