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Hexose transport and membrane depolarization in Riccia fluitans.

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This study reveals two distinct pathways for 3-O-methyl glucose (3-OMG) uptake in Riccia fluitans: one pH-dependent and saturable, the other pH-insensitive and linear. Hexoses also depolarize cell membranes, suggesting a dual-mode hexose carrier mechanism.

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Area of Science:

  • Plant Physiology
  • Membrane Transport
  • Biochemistry

Background:

  • The aquatic liverwort Riccia fluitans is a model organism for studying plant cell transport mechanisms.
  • Understanding hexose uptake is crucial for plant metabolism and growth.

Purpose of the Study:

  • To investigate the kinetics and mechanisms of 3-O-methyl glucose (3-OMG) uptake in Riccia fluitans.
  • To analyze the effects of hexoses on membrane potential and electrical conductance.

Main Methods:

  • Utilized (14)C-labeled 3-OMG to study uptake rates over time and varying concentrations.
  • Measured membrane depolarization (ΔΨ m) in response to different hexoses.
  • Investigated the influence of pH, K(+), and H(+) on hexose transport.

Main Results:

  • 3-OMG uptake exhibited a pH-dependent, saturable component (Km ≈ 0.1 mM) and a pH-insensitive, linear component.
  • Hexoses caused rapid plasmalemma depolarization, with varying half-maximal concentrations (e.g., glucose 0.24 mM, fructose 34 mM).
  • Electrical conductance of the thallus cell membrane increased upon hexose exposure.

Conclusions:

  • A hexose carrier model is proposed, operating either electrogenically (protonated, pH/voltage-sensitive) or via transmembrane diffusion of its uncharged form.
  • The findings suggest a complex, dual-mode mechanism for hexose transport in Riccia fluitans.