Related Experiment Videos
Granulocyte colony stimulating activity derived from human keratinocytes.
Lymphokine Research
|January 1, 1986
Summary
Human keratinocytes produce factors that stimulate blood cell growth. These keratinocyte-derived factors, including colony-stimulating factors (CSFs), play a role in skin inflammation and tumor development.
Area of Science:
- Dermatology
- Hematology
- Immunology
Background:
- Human keratinocytes secrete soluble mediators, including epidermal cell-derived thymocyte activating factor (ETAF), similar to interleukin-1 (IL-1).
- Skin cells are implicated in localized inflammatory and neoplastic responses.
Purpose of the Study:
- To investigate the production of hemopoietic colony-stimulating factors (CSFs) by human keratinocytes.
- To characterize the effects of keratinocyte-derived mediators on hemopoietic progenitor cells.
Main Methods:
- Keratinocyte supernatants (normal and cancer cell lines) were purified using ultrafiltration, Sephadex G-100 chromatography, and HPLC.
- Purified fractions were tested for their effects on human peripheral blood hemopoietic progenitors in methylcellulose cultures.
- Interleukin-3 (IL-3)-like activity was assessed using a murine IL-3-dependent cell line.
Main Results:
- Keratinocyte supernatants stimulated the growth and differentiation of granulocyte progenitor cells (GM- and Eo-type CFU-c).
- Supernatants exhibited IL-3-like activity.
- HPLC separation revealed a dose-response relationship for GM- and Eo-colony stimulation and dissociated ETAF from CSF activity.
Conclusions:
- Human keratinocytes produce hemopoietic CSFs.
- These findings support the role of keratinocytes and their products in skin inflammatory and neoplastic processes.