Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using community-based participatory research to contextualize Latino exposure to community violence: A mixed qualitative and spatial analysis approach.

American journal of community psychology·2025
Same author

Referrals to child and family services during the COVID-19 pandemic: An analysis of administrative data from British Columbia and Northwest Territories, Canada.

Child abuse & neglect·2025
Same author

Associations between hysterectomy and pelvic floor disorders: a systematic review and meta-analysis.

American journal of obstetrics and gynecology·2025
Same author

Neurological sequelae of long COVID: a comprehensive review of diagnostic imaging, underlying mechanisms, and potential therapeutics.

Frontiers in neurology·2025
Same author

BDNF-dependent nano-organization of Neogenin and the WAVE regulatory complex promotes actin remodeling in dendritic spines.

iScience·2024
Same author

On-demand fabrication of piezoelectric sensors for in-space structural health monitoring.

Smart materials & structures·2024

Related Experiment Video

Updated: May 5, 2026

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM
09:25

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

11.0K

RGMa regulates cortical interneuron migration and differentiation.

Conor O'Leary1, Stacey J Cole, Michael Langford

  • 1The University of Queensland, Queensland Brain Institute, Brisbane, Queensland, Australia.

Plos One
|December 7, 2013
PubMed
Summary
This summary is machine-generated.

Repulsive Guidance Molecule alpha (RGMa) prevents interneuron re-entry into the ganglionic eminence ventricular zone during brain development. RGMa-Neogenin interactions guide interneuron migration, crucial for preventing neuropsychiatric disorders.

More Related Videos

Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence MGE Cells for In Vivo Studies
09:21

Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence MGE Cells for In Vivo Studies

Published on: April 23, 2015

10.9K
Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
09:10

Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy

Published on: May 12, 2015

11.1K

Related Experiment Videos

Last Updated: May 5, 2026

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM
09:25

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

11.0K
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence MGE Cells for In Vivo Studies
09:21

Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence MGE Cells for In Vivo Studies

Published on: April 23, 2015

10.9K
Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
09:10

Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy

Published on: May 12, 2015

11.1K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Neuropsychiatric disorders like schizophrenia and autism are linked to impaired neocortex development and neural network formation.
  • Cortical inhibitory interneurons, originating from the medial ganglionic eminence (MGE), migrate to the embryonic cortex, a process guided by chemoattractants and chemorepellents.
  • The cues preventing interneuron re-entry into the ganglionic eminence ventricular zone remain poorly understood.

Purpose of the Study:

  • To investigate the role of Repulsive Guidance Molecule alpha (RGMa) in regulating interneuron migration during neocortex development.
  • To elucidate the mechanism by which RGMa prevents interneurons from re-entering the ventricular zone of the ganglionic eminences.
  • To explore the interaction between RGMa, its receptor Neogenin, and other guidance cues like Netrin-1 in directing interneuron migration.

Main Methods:

  • In vitro assays to assess RGMa's effect on interneuron differentiation and neurite outgrowth.
  • In vitro explant and migration assays to evaluate RGMa's repulsive guidance properties for newborn interneurons.
  • Simultaneous exposure of interneurons to RGMa and Netrin-1 gradients to investigate potential regulatory mechanisms.

Main Results:

  • RGMa promotes interneuron differentiation and neurite outgrowth in vitro.
  • RGMa acts as a repulsive guidance cue, preventing newborn MGE-derived interneurons from migrating back into the ventricular zone.
  • Netrin-1 alone did not affect interneuron migration, but co-exposure with RGMa abrogated RGMa-induced chemorepulsion, suggesting a novel regulatory interaction.

Conclusions:

  • RGMa-Neogenin interactions are crucial for directing interneuron migration out of the ganglionic eminence ventricular zone.
  • RGMa functions as a key repulsive cue preventing interneuron re-entry into the ventricular zone, thereby establishing a migratory corridor.
  • The interplay between RGMa and Netrin-1 offers a novel mechanism for tightly regulating interneuron migration during peak neurogenesis.