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Updated: May 5, 2026

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

Published on: April 3, 2011

Proteomic interactions in the mouse vitreous-retina complex.

Jessica M Skeie¹, Vinit B Mahajan

¹Omics Laboratory, University of Iowa, Iowa City, Iowa, United States of America ; Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa, United States of America.

|December 7, 2013

Summary

This study identified proteins in the mouse vitreous and retina, revealing pathways for oxidative stress and immune reactions. These findings offer insights into vitreoretinal diseases and potential biomarkers for diagnosis and therapy.

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Area of Science:

Proteomics
Ophthalmology
Biochemistry

Background:

Human vitreoretinal diseases are poorly understood due to limited translational models.
Abnormal mechanical interactions between the vitreous and retina are implicated in disease pathogenesis.

Purpose of the Study:

To determine the proteomic profile of the normal mouse vitreous and retina.
To identify nonstructural proteins and potential biomarkers in the mouse vitreous and retina for translational research.

Main Methods:

Vitreous and retinal samples were collected from mice.
Liquid chromatography-tandem mass spectrometry was used for protein identification.
Bioinformatic software analyzed protein expression, differential expression, and functional interactions.

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Last Updated: May 5, 2026

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

Published on: April 3, 2011

Dissection of Human Vitreous Body Elements for Proteomic Analysis

Dissection of Human Vitreous Body Elements for Proteomic Analysis

Published on: January 23, 2011

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

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Published on: November 12, 2017

Main Results:

1,680 unique proteins were identified in the retina and 675 in the vitreous.
Distinct pathways were found in retina (oxidative phosphorylation, neurofilament remodeling) and vitreous (oxidative stress, innate immunology).
Potential biomarkers for human diseases, including carbonic anhydrases, crystallins, and glutathione peroxidase, were identified in mouse samples.

Conclusions:

The mouse vitreous expresses nonstructural proteins involved in oxidative stress and immune responses, interacting with the retina.
Intracellular proteins may be exchanged between the retina and vitreous.
This proteomic dataset and identified biomarkers can advance the study of human vitreoretinopathies in mouse models.