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Increased adiposity in annexin A1-deficient mice
Rand T Akasheh1, Maria Pini, Jingbo Pang
1Department of Kinesiology and Nutrition, University of Illinois at Chicago, Chicago, Illinois, United States of America.
Plos One
|December 7, 2013
Summary
Annexin A1 (ANXA1) protein influences body fat regulation. ANXA1 knockout mice showed increased adiposity and insulin resistance, indicating its crucial role in managing obesity.
Area of Science:
- Metabolic research
- Obesity research
- Molecular endocrinology
Background:
- Annexin A1 (ANXA1) is a protein involved in glucocorticoid-mediated anti-inflammatory actions.
- ANXA1's role in regulating adiposity and its modulation in obesity remain largely uninvestigated.
- Altered ANXA1 production is observed in obesity, prompting further research into its function.
Purpose of the Study:
- To investigate the modulation of ANXA1 in adipose tissue during obesity in murine models.
- To determine the specific involvement of ANXA1 in the development of diet-induced obesity.
- To elucidate the functional consequences of ANXA1 deficiency on metabolic parameters.
Main Methods:
- Utilized high-fat diet (HFD) induced obesity models in C57BL6 and Balb/c mice.
- Employed leptin-deficient (ob/ob) and IL-6 knockout (KO) mice to assess ANXA1 regulation independence.
- Analyzed ANXA1 knockout (ANXA1 KO) mice, comparing them to wild-type (WT) counterparts under HFD conditions.
- Measured body weight, fat mass, leptin, adipocyte size, lipolytic enzyme expression, glucose/insulin levels, and corticosterone.
Main Results:
- ANXA1 mRNA was significantly upregulated in adipose tissue of HFD-fed mice, independent of leptin or IL-6.
- Female Balb/c ANXA1 KO mice on HFD exhibited increased adiposity, body weight, fat mass, leptin levels, and adipocyte size compared to WT mice.
- ANXA1 KO mice on HFD displayed impaired lipolytic pathway activation, elevated fasting glucose and insulin, and developed insulin resistance.
- ANXA1 KO mice showed elevated plasma corticosterone and blunted downregulation of 11-beta hydroxysteroid dehydrogenase type 1, without altered adipose tissue inflammation markers.
Conclusions:
- ANXA1 is a significant modulator of adiposity in mice.
- Female ANXA1 KO mice are more susceptible to weight gain and diet-induced insulin resistance.
- ANXA1 deficiency impacts metabolic regulation, leading to increased adiposity and insulin resistance without affecting adipose tissue inflammation.
