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Updated: May 5, 2026
Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
Published on: March 24, 2023
Determination of comparative minimum inhibitory concentration (MIC) of bacteriocins produced by enterococci for
Maryam Hassan1, Yousef Javadzadeh, Farzaneh Lotfipour
1Faculty of Pharmacy and Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Introduction:
The occurrence of multi-antibiotic resistance among enterococci is a prevalent clinical problem worldwide and continues to get serious due to the lack of efficient therapeutic options by the time. In this regards, prokaryotic antimicrobial peptides with bactericidal or bacteriostatic activity which are directed against bacterial strains closely related to producer strains looks one of the promising alternative to conventional antibiotics.
Methods:
The antibiotic susceptibility pattern of 20 clinical isolates of enterococci was evaluated and subsequently the isolates were screened for antibacterial activity against three different indicator strains. The efficacy of potential bacteriocinogenic isolates were assayed against multi-antibiotic resistant Enterococcus spp. by comparative minimum inhibitory concentration (MIC).
Results:
Antibiotic resistant pattern of enterococcal isolates demonstrated that multi-antibiotic resistant to conventional antibiotics were significantly high and the prevalent pattern of resistance was combination of gentamicin, streptomycin, chloramphenicol and vancomycin. In addition, the data from comparative MIC showed the noticeable activity of selected potential bacteriocinogenic strains against pathogenic enterococci.
Conclusion:
The present survey may address the potential applicability of antimicrobial peptides in alleviating the problems of antibiotic resistance.
Insights
Multi-antibiotic resistant enterococci pose a global health threat. Prokaryotic antimicrobial peptides show promise as alternatives to conventional antibiotics, demonstrating notable activity against resistant strains.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Multi-antibiotic resistance in enterococci is a significant worldwide clinical challenge.
- Limited effective therapeutic options exacerbate the problem of enterococcal infections.
- Prokaryotic antimicrobial peptides (bacteriocins) offer a potential alternative to conventional antibiotics.
Purpose of the Study:
- To evaluate the antibiotic susceptibility patterns of clinical enterococcal isolates.
- To screen these isolates for antibacterial activity against indicator strains.
- To assess the efficacy of bacteriocinogenic isolates against multi-antibiotic resistant Enterococcus spp.
Main Methods:
- Evaluated antibiotic susceptibility of 20 clinical enterococcal isolates.
- Screened isolates for antibacterial activity against three indicator strains.
- Determined Minimum Inhibitory Concentration (MIC) of bacteriocinogenic isolates against resistant Enterococcus spp.
Main Results:
- A high prevalence of multi-antibiotic resistance was observed in enterococcal isolates.
- Common resistance patterns included combinations of gentamicin, streptomycin, chloramphenicol, and vancomycin.
- Selected bacteriocinogenic strains exhibited significant inhibitory activity against pathogenic enterococci.
Conclusions:
- Antimicrobial peptides derived from prokaryotes show potential for combating antibiotic resistance.
- This study highlights the applicability of bacteriocins as a strategy against multi-drug resistant enterococci.
