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Degenerative effect of Cisplatin on testicular germinal epithelium
Daryosh Mohammadnejad1, Ali Abedelahi, Jafar Soleimani-Rad
1Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Purpose:
The present study was designed to explore the effect of intraperitoneal administration of cisplatin in germinal epithelium of mice. There are few reports on the side effect of cisplatin on spermatogenesis when are used as anticancer drug. Therefore, in the present study the effect of cisplatin on spermatogenesis was evaluated by electron microscopy.
Methods:
Twenty balb/c mice aging 6-8 weeks was used in this study. The mice were divided into two groups, control and cysplatin treated. cysplatin was injected for five days as 2.5 mg /kg. The mice were sacrificed after 5 weeks and testicular specimens were removed, fixed in boueins, formaldeyd fixative and 2.5% Glutaraldehide then prepared for light and electron microscopic study.
Results:
Observation with optic microscope in treated group thickness of germinal epithelium was reduced a lot and increased the number of apoptotic cells. In some seminiferous tubules only sertoli cells were observed and nucleus of spermatogony cells was hetrochromatin. The electron microscopic observations showed some irregularity waviness and thickening in basal layer. Also myoid cells of this group were thick and contracted. In this group many apoptotic cells and damaged organelles were seen.
Conclusion:
It was indicated that cisplatin affected testicular germinal epithelium by both cytotoxic effect and induction of apoptosis.
Insights
Cisplatin significantly damages the germinal epithelium in mice testes, leading to reduced thickness and increased cell death. This anticancer drug impacts spermatogenesis through cytotoxic effects and apoptosis induction.
Area of Science:
- Reproductive Biology
- Toxicology
- Cell Biology
Background:
- Cisplatin is a widely used anticancer drug.
- Limited data exists on cisplatin's specific side effects on spermatogenesis.
- Understanding these effects is crucial for managing treatment outcomes.
Purpose of the Study:
- To investigate the impact of intraperitoneal cisplatin administration on the germinal epithelium of mouse testes.
- To evaluate the effects of cisplatin on spermatogenesis using electron microscopy.
Main Methods:
- Balb/c mice (6-8 weeks old) were divided into control and cisplatin-treated groups.
- Cisplatin was administered intraperitoneally at 2.5 mg/kg for five days.
- Testicular tissues were analyzed using light and electron microscopy after five weeks.
Main Results:
- Cisplatin treatment reduced germinal epithelium thickness and increased apoptotic cells.
- Observed abnormalities included heterochromatin in spermatogony nuclei and Sertoli cell-only tubules.
- Electron microscopy revealed basal layer irregularities, thickened myoid cells, damaged organelles, and widespread apoptosis.
Conclusions:
- Cisplatin exerts cytotoxic effects on testicular germinal epithelium.
- Cisplatin induces apoptosis in germinal cells, impacting spermatogenesis.
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