Gene expression in Mammalian cells and its applications

Area of Science:

• Biotechnology
• Molecular Biology
• Cell Biology

Background:

• Protein production requires high quantity and quality, driving increased use of mammalian cells.
• Mammalian expression systems facilitate proper protein folding, post-translational modifications, and assembly for biological activity.

Purpose of the Study:

• To raise awareness among researchers about gene expression in mammalian cells.
• To provide an overview of molecular technologies, expression systems, and applications in mammalian cell lines.

Main Methods:

• Literature survey focusing on mammalian expression systems.
• Exploration of various mammalian cell lines for gene expression.
• Review of gene transfer vector systems including plasmid, adenovirus, vaccinia, retroviral, and baculovirus vectors.
• Examination of gene transfer processes into mammalian cells.

Main Results:

• Detailed analysis of different mammalian cell lines used for gene expression.
• Comprehensive review of diverse gene transfer vectors and their mechanisms.
• Discussion of the applications and limitations inherent in mammalian expression systems.

Conclusions:

• Mammalian expression systems are vital for producing complex recombinant proteins.
• Understanding these systems, vectors, and applications is key for advancing biopharmaceutical development.
• This review serves as a foundational resource for researchers entering the field of mammalian gene expression.