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Related Experiment Video

Updated: May 5, 2026

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Construction of pPIC9 Recombinant Vector Containing Human Stem Cell Factor.

Behrooz Farhadi1, Mahmoud Shekari Khaniani, Sima Mansoori Derakhshan

  • 1Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran.

Advanced Pharmaceutical Bulletin
|December 7, 2013
PubMed
Summary

Researchers constructed a recombinant vector containing human stem cell factor (SCF) for potential large-scale production. This work advances the use of SCF in therapies like stem cell expansion and immunotherapy.

Keywords:
CloningExpressionHuman SCFPichia pastoris

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Hematopoiesis Research

Background:

  • Cytokines regulate critical stem cell functions including proliferation and differentiation.
  • Stem cell factor (SCF) is a hematopoietic cytokine crucial for stem cell biology.
  • SCF has therapeutic potential in stem cell mobilization, expansion, gene therapy, and immunotherapy.

Purpose of the Study:

  • To construct a pPIC9 recombinant vector encoding human SCF (hSCF).
  • To facilitate the production of recombinant human SCF (rhSCF) for therapeutic applications.

Main Methods:

  • Human SCF cDNA was amplified using PCR.
  • hSCF cDNA and pPIC9 vector were digested with EcoR I and Xho I restriction enzymes.
  • Ligation, PCR, and sequence analysis were performed to verify the recombinant vector.

Main Results:

  • The successful construction of the pPIC9 recombinant vector containing hSCF cDNA was confirmed via sequencing.
  • The recombinant vector is suitable for transformation into yeast expression systems.

Conclusions:

  • The rhSCF/pPIC9 vector enables the use of Pichia pastoris yeast as a eukaryotic host for industrial-scale human SCF production.
  • This method provides a foundation for scalable rhSCF manufacturing for therapeutic use.