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Forensic signatures for Marburgviruses.

Jian Song1, Murray Wolinsky, Melinda Wren

  • 1Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, United States.

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|December 10, 2013
PubMed
Summary

Forensic assays were developed for Marburgvirus, a dangerous hemorrhagic fever virus. These assays enable accurate identification and genotyping of Marburgvirus strains for forensic investigations.

Keywords:
Canonical SNPsForensic scienceHemorrhagic fever virusMarburgvirusSNP genotypingTaqMan-MGB allelic discrimination assay

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Area of Science:

  • Virology
  • Forensic Science
  • Molecular Biology

Background:

  • Marburgvirus is a highly infectious and fatal hemorrhagic fever virus.
  • It poses a significant biothreat due to easy transmission, including respiratory routes.
  • Existing detection assays lack forensic application capabilities.

Purpose of the Study:

  • To develop and validate high-resolution forensic assays for Marburgvirus.
  • To enable accurate genotyping of Marburgvirus strains and outbreaks for forensic analysis.

Main Methods:

  • Phylogenetic analysis of Marburgvirus strains and isolates.
  • Identification of canonical single nucleotide polymorphisms (SNPs) for clades and strains.
  • Design and screening of TaqMan-MGB allelic discrimination assays targeting identified SNPs.

Main Results:

  • Developed 45 validated forensic assays with 100% clade coverage and 91% strain coverage.
  • Assays demonstrated robustness with built-in redundancy.
  • Successfully performed accurate forensic analysis on three unknown Marburgvirus samples.

Conclusions:

  • The developed Marburgvirus forensic assays provide high-resolution genotyping capabilities.
  • These assays facilitate rapid and accurate identification crucial for forensic investigations.
  • The assays represent a significant advancement in Marburgvirus forensic analysis.