Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Wood Surfacing01:14

Wood Surfacing

493
Wood surfacing is a critical finishing process designed to smoothen the wood surface, enhance its dimensional accuracy, and make handling safer. This process compensates for potential shrinkage during the seasoning phase by marginally increasing the wood dimensions before surfacing. It also helps correct some distortions that may occur as the wood dries.
The equipment used in the surfacing process is a plane equipped with rotating blades. This tool efficiently smoothens the wood surface and can...
493
Aliasing01:18

Aliasing

945
Accurate signal sampling and reconstruction are crucial in various signal-processing applications. A time-domain signal's spectrum can be revealed using its Fourier transform. When this signal is sampled at a specific frequency, it results in multiple scaled replicas of the original spectrum in the frequency domain. The spacing of these replicas is determined by the sampling frequency.
If the sampling frequency is below the Nyquist rate, these replicas overlap, preventing the original...
945
Shape and Texture of Coarse Aggregate01:25

Shape and Texture of Coarse Aggregate

1.3K
Aggregate shape is classified based on the relative sharpness or roundness of the edges and corners. This classification includes categories like rounded, angular, elongated, and flaky, each with specific characteristics. Rounded aggregates, fully shaped by attrition, are typical of river or seashore gravel, while angular aggregates, such as crushed rock, have well-defined edges. Aggregates that are elongated and flaky are less desirable, as they can reduce the workability and strength of...
1.3K
Reducing Line Loss01:18

Reducing Line Loss

524
In a three-phase circuit, line loss is an indicator of energy dissipated as heat due to the resistance of transmission lines. To address this, incorporating transformers into the system—a step-up transformer at the source and a step-down transformer at the load—is a strategic solution. Two three-phase transformers are introduced to improve this.
With a step-up transformer at the source, the voltage is increased, thereby reducing the current in the transmission lines since power loss...
524
Design Example: Aggregate Gradation01:24

Design Example: Aggregate Gradation

470
The right type and quality of aggregates are crucial for concrete as they significantly influence its properties, mix proportions, and cost-effectiveness. If different sources are available for sand, the commonly used fine aggregate in concrete, the selection of sand is primarily based on its gradation.
The grading, or particle-size distribution, of sand is determined using sieve analysis, with standard sizes ranging from 150 μm to 10 mm (ASTM No. 100 sieve to 3⁄8 in. sieve). Sand is...
470
Quarrying of Stone01:15

Quarrying of Stone

855
Quarrying is the process of extracting stone from a quarry, where specialized techniques are employed to remove large blocks of stone safely and efficiently. This process can involve controlled explosions or more precision-oriented methods such as cutting and drilling.
One common method involves using a diamond belt saw to cut large blocks from the quarry face. These blocks can be about 50 feet long and 12 feet high. After the initial vertical cut, drilling is performed at the base of the...
855

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CCL19<sup>+</sup> fibroblasts define a proliferative niche in chronic lymphocytic leukemia.

bioRxiv : the preprint server for biology·2026
Same author

High-throughput biochemical phenotyping of SHP2 variants reveals the molecular basis of diseases and allosteric drug inhibition.

bioRxiv : the preprint server for biology·2026
Same author

Adhesion G protein-coupled receptors.

Pharmacological reviews·2026
Same author

Activating Ras-MAPK pathway variants drive hippocampal clonal competition in human epilepsy.

bioRxiv : the preprint server for biology·2026
Same author

Design of soluble Notch agonists that drive T cell development and boost immunity.

Cell·2025
Same author

β-arrestin recruitment facilitates a direct association with G proteins.

bioRxiv : the preprint server for biology·2025
Same journal

Structure of Perinereis linea erythrocruorin reveals a compact extracellular globin megacomplex.

Structure (London, England : 1993)·2026
Same journal

Meet the author: Stephen Brohawn.

Structure (London, England : 1993)·2026
Same journal

Tetraspanins bring Norrin into focus: Structural insights into ligand-specific Wnt signaling.

Structure (London, England : 1993)·2026
Same journal

Uncovering subtype-selective activation of the K<sub>Ca</sub>3.1 channel by SKA-111.

Structure (London, England : 1993)·2026
Same journal

Identification and structure determination of a type III-Bv CRISPR complex that post-translationally modifies an associated toxin.

Structure (London, England : 1993)·2026
Same journal

Cryo-EM structure of the Arabidopsisthaliana ribosome in translating and non-translating states.

Structure (London, England : 1993)·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

Convergent Polishing: A Simple, Rapid, Full Aperture Polishing Process of High Quality Optical Flats & Spheres
13:07

Convergent Polishing: A Simple, Rapid, Full Aperture Polishing Process of High Quality Optical Flats & Spheres

Published on: December 1, 2014

10.2K

Refining a Jagged edge.

Stephen C Blacklow1

  • 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 45 Shattuck Street, Boston, MA 02115, USA; Department of Cancer Biology, Dana Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA; Department of Pathology, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, USA.

Structure (London, England : 1993)
|December 10, 2013
PubMed
Summary
This summary is machine-generated.

The Notch ligand Jagged1's N-terminal MNNL domain requires calcium for signal transduction. This domain structurally resembles a C2 domain, crucial for Notch pathway activity.

More Related Videos

Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes
08:32

Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes

Published on: June 30, 2019

7.4K
Impact of Fabrication Techniques and Polishing Procedures on Surface Roughness of Denture Base Resins
03:02

Impact of Fabrication Techniques and Polishing Procedures on Surface Roughness of Denture Base Resins

Published on: January 17, 2025

1.2K

Related Experiment Videos

Last Updated: May 5, 2026

Convergent Polishing: A Simple, Rapid, Full Aperture Polishing Process of High Quality Optical Flats & Spheres
13:07

Convergent Polishing: A Simple, Rapid, Full Aperture Polishing Process of High Quality Optical Flats & Spheres

Published on: December 1, 2014

10.2K
Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes
08:32

Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes

Published on: June 30, 2019

7.4K
Impact of Fabrication Techniques and Polishing Procedures on Surface Roughness of Denture Base Resins
03:02

Impact of Fabrication Techniques and Polishing Procedures on Surface Roughness of Denture Base Resins

Published on: January 17, 2025

1.2K

Area of Science:

  • Cellular biology
  • Molecular biology
  • Biochemistry

Background:

  • The Notch signaling pathway is crucial for cell-to-cell communication and development.
  • Jagged1 is an essential ligand in the Notch pathway, mediating signal transduction.
  • Understanding the structural and functional properties of Jagged1 is key to deciphering Notch signaling.

Purpose of the Study:

  • To investigate the structural and functional characteristics of the N-terminal MNNL domain of Jagged1.
  • To determine the role of calcium binding in the activity of the MNNL domain.
  • To elucidate the mechanism by which Jagged1 facilitates Notch signal transduction.

Main Methods:

  • Structural analysis of the MNNL domain.
  • Calcium binding assays.
  • Functional assays to measure Notch signal transduction.

Main Results:

  • The N-terminal MNNL domain of Jagged1 structurally resembles a C2 domain.
  • Calcium binding is essential for the MNNL domain's function.
  • The MNNL domain's calcium-dependent activity is critical for productive Notch signal transduction.

Conclusions:

  • The MNNL domain of Jagged1 acts as a calcium-dependent module.
  • This calcium-binding C2-like domain is vital for Jagged1's role in Notch signaling.
  • Findings provide insights into the molecular mechanisms of Notch ligand function.