Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mechanically-gated Ion Channels

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Expression and functional characterization of the TRPV3 channel in domestic cat (Felis catus) mature oocytes.

Biological research·2026

Same author

A phenotypic <i>in vitro</i> assay of mouse oocyte maturation identifies reversible blockers of meiotic progression for non-hormonal contraceptive discovery.

Human reproduction open·2026

Same author

Biocompatible designated Resin-3D-printed polymers exhibit reproductive toxicity prevented by Parylene-C.

bioRxiv : the preprint server for biology·2026

Same author

Expression of purinergic receptor P2Y, G-protein coupled 2, is regulated by estrogen during the estrous cycle in mouse uterus.

Purinergic signalling·2026

Same author

Phenotypic CRISPR screens identify NLRX1 as an essential activator of the human mitochondrial permeability transition.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Estrogen-Dependent Regulation of FDPS in the Mouse Uterus and Its Expression in Endometrial Cancer.

International journal of molecular sciences·2026

Search research articles

Related Experiment Video

Updated: May 5, 2026

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

TRPV3 channels mediate strontium-induced mouse-egg activation.

Ingrid Carvacho¹, Hoi Chang Lee², Rafael A Fissore²

¹Howard Hughes Medical Institute, Department of Cardiology, Boston Children's Hospital, Boston, MA 02115, USA; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.

|December 10, 2013

Summary

Transient Receptor Potential Vanilloid 3 (TRPV3) channels mediate calcium influx essential for mammalian egg activation. These TRPV3 channels are crucial for strontium-induced egg activation and embryonic development.

More Related Videos

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Controllable Ion Channel Expression through Inducible Transient Transfection

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

Related Experiment Videos

Last Updated: May 5, 2026

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Controllable Ion Channel Expression through Inducible Transient Transfection

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

Area of Science:

Reproductive Biology
Ion Channel Physiology
Developmental Biology

Background:

Calcium influx is critical for mammalian oocyte maturation and egg activation.
The specific ion channels responsible for this calcium influx remain largely unidentified.

Purpose of the Study:

To identify the molecular identity of calcium-permeant channels involved in initiating embryonic development.
To investigate the role of TRPV3 in oocyte maturation, egg activation, and strontium-induced activation.

Main Methods:

Electrophysiological recordings of ion channel currents in oocytes.
Analysis of oocyte maturation and activation in wild-type and TrpV3 knockout mouse models.
Assessment of strontium ion (Sr(2+)) influx and activation in TrpV3(-/-) eggs.

Main Results:

A novel transient receptor potential (TRP) ion channel current, identified as TRPV3, was characterized.
TRPV3 expression and activity peak at metaphase of meiosis II (MII), the stage of fertilization.
TRPV3 activation mediates significant calcium entry, inducing egg activation.
TrpV3 knockout eggs lack strontium influx and fail to activate in response to strontium.

Conclusions:

TRPV3 is a key mediator of calcium influx in mouse oocytes.
TRPV3 is essential for strontium-induced egg activation.
TRPV3 represents a potential target for artificial egg activation strategies.