Related Experiment Video
Updated: May 5, 2026
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
Published on: February 12, 2015
New experimental model of exposure to environmental tobacco smoke
Purpose:
To describe a new model to passive smoking for rodents.
Methods:
Twenty rats were distributed into two study groups (N=10): control group (CG), that was not exposed to tobacco smoke and used as normal standard for biochemical and histological analysis; Experimental Group (EG), that Animals were exposed to the passive smoking; Euthanasia was performed after 14 days of exposure. The serum level of nicotine and histological analysis were performed.
Results:
There was a statistical difference on the nicotine serum levels between Experimental and Control group, with level of 286 ± 23 nanograma/mL in the EG and undetectable on CG (p<0.01). The histological study suggested the model efficacy producing alveolar destruction and emphysema in the EG compared with the insignificant lesions in the CG's lung.
Conclusion:
The model of exposure to environmental tobacco smoke for rodents induced easily the changes related to secondhand smoke.
