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Updated: Feb 8, 2026

Minimum Burning Pressures of Water-based Emulsion Explosives
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Nanobioprobe mediated DNA aptamers for explosive detection.

Priyanka, Munish Shorie, Vijayender Bhalla

    Chemical Communications (Cambridge, England)
    |December 10, 2013
    PubMed
    Summary

    Researchers developed specific DNA aptamers to detect the explosive trinitrotoluene (TNT). These aptamers enable a new electrochemical biosensor with a detection limit of 1 part per billion for TNT detection.

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    Area of Science:

    • Analytical Chemistry
    • Biotechnology
    • Materials Science

    Background:

    • Explosive detection remains a critical challenge in security and environmental monitoring.
    • Nucleic acid aptamers offer a promising alternative to traditional detection methods due to their specificity and stability.
    • Developing rapid and sensitive detection methods for hazardous materials like trinitrotoluene (TNT) is essential.

    Purpose of the Study:

    • To develop specific nucleic acid aptamers against the explosive trinitrotoluene (TNT).
    • To create a novel electrochemical aptasensor for sensitive TNT detection.
    • To evaluate the performance of the developed aptasensor for practical applications.

    Main Methods:

    • Modified SELEX (Systematic Evolution of Ligands by Exponential Enrichment) method using microtiter plates for aptamer selection.
    • Streptavidin-labeled gold nanoprobes for efficient partitioning of double-stranded DNA (dsDNA).
    • Development of an electrochemical aptasensor utilizing the selected TNT-specific aptamers.

    Main Results:

    • Successfully selected specific DNA aptamers with high affinity (~10(-7) M) for TNT.
    • The developed electrochemical aptasensor demonstrated a low detection limit of approximately 1 part per billion (ppb) for TNT.
    • The aptasensor showed efficient partitioning of dsDNA through streptavidin-labeled gold nanoprobes during selection.

    Conclusions:

    • The study reports the successful development of specific aptamers against TNT using a modified SELEX approach.
    • A novel electrochemical aptasensor based on these aptamers provides sensitive and specific detection of TNT.
    • The findings highlight the potential of aptasensor technology for rapid and reliable explosive detection.