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Updated: May 5, 2026

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
Published on: March 15, 2018
Protein synthesis and auxin-induced growth: Inhibitor studies
1Botany Department, University of Washington, 98195, Seattle, WA, USA.
Abstract:
We have compared the effects of cycloheximide (CHI) and two other rapid and effective inhibitors of protein synthesis, pactamycin and 2-(4-methyl-2,6-dinitroanilino)-N-methyl proprionamide (MDMP), on protein synthesis, respiration, auxin-induced growth and H(+)-excreation of Avena sativa L. coleoptiles. All three compounds inhibit protein synthesis without affecting respiration. The effectiveness of the inhibitors against H(+)-excretion and growth correlates with their ability to inhibit protein synthesis. Both CHI and MDMP inhibit auxin-induced H(+)-excretion after a latent period of 5-8 min, and inhibit growth after a 8-10-min lag. These results support the idea that continued protein synthesis is required in the initial stages of the growth-promoting action of auxin.
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