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Characterization of the rDNA repeat units in the MitchellPetunia genome
J Waldron1, P Dunsmuir, J Bedbrook
1CSIRO Division of Plant Industry, P. O. Box 1600, 2601, Canberra City, A.C.T., Australia.
Abstract:
The principal rDNA repeating unit in the MitchellPetunia genome has a length of 8.5 kb. In addition there is a major variant of length 9.7 kb, and two minor variants of 9.3 kb and 10.4 kb. The size heterogeneity of the rDNA repeating units results from length differences in the non-transcribed spacer regions. These differences may reflect simple insertions into the non-transcribed spacer region of the major 'short' repeat; however, additional sequence changes have occurred since the 'short' repeat is characterized by restriction endonuclease cleavage sites which are absent in the longer variant units.
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