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Tests on protection against viral diseases.
Comparative Immunology, Microbiology and Infectious Diseases
|January 1, 1986
Summary
Animal models remain crucial for evaluating Biological Response Modifiers (BRMs) against viral infections. This study highlights their effectiveness in assessing BRM potency against Pseudorabies and Vesicular Stomatitis viruses.
Area of Science:
- Immunology
- Virology
- Pharmacology
Background:
- In vitro methods for assessing Biological Response Modifiers (BRMs) efficacy are limited.
- Animal challenge models are essential for evaluating the comprehensive defense mechanisms against viral diseases.
Purpose of the Study:
- To develop and compare two distinct mouse viral challenge models for assessing BRM efficacy.
- To correlate in vitro measured immune responses with in vivo protection against viral infections.
Main Methods:
- Development of Pseudorabies virus (PRV) and Vesicular Stomatitis virus (VSV) challenge models in NMRI mice.
- Administration of various BRMs via different routes and pretreatment times.
- In vitro (ex vivo) assessment of antiviral defense mechanisms in infected animals.
Main Results:
- BRM efficacy varied significantly based on BRM type, administration route, and timing.
- In vitro assessed defense mechanisms against PRV correlated well with in vivo protection.
- Comparative analysis revealed differences in BRM potency across distinct viral challenge models.
Conclusions:
- Selective viral challenge models combined with in vitro immune function screening offer reliable methods to evaluate BRM potency.
- Animal models are indispensable for a holistic assessment of BRMs' protective effects against viral infections.