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Updated: May 5, 2026

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
Published on: February 3, 2023
Normal-phase HPLC quantitation of chlorophyll a' and phylloquinone in Photosystem I particles
H Maeda1, T Watanabe, S Kobayashi
1Institute of Industrial Science, University of Tokyo, Roppongi, Minato-ku, 106, Tokyo, Japan.
Abstract:
Fractionated Photosystem (PS) I particles consisting of six, five or two core proteins were analyzed by HPLC for chlorophyll (Chl) a' and phylloquinone (PhQ). Each particle had a Chl a/P700 molar ratio of 50-55 and contained ca. 2 molecules of Chl a' per P700. Deliberate control of eluent composition led to isolated elution of PhQ and β-carotene in the normal-phase chromatogram. Based on these a simple HPLC procedure has been established to determine the PhQ/P700 molar ratio, which was ca. 2 for the larger two PS I particles and ca. 1 for the smallest particle, in line with previous reports.
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