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Ginkgo biloba extract reducing myocardium cells apoptosis by regulating apoptotic related proteins expression in
Zeng Yong Qiao1, Jian Hua Huang, Jiang Wei Ma
1Department of Cardiology, Fengxian Branch of Shanghai 6th People's Hospital, Nanfeng road 6600#, Shanghai, 201400, China.
Abstract:
The Bax, cyt-c and caspase-3 proteins play an important role in regulating the myocardial apoptosis. Although very little is known about the specific signal pathways modulated by Ginkgo biloba extract (GBE), it seems advisable to suppose that GBE-induced antiapoptotic effect might be attributed to the regulation of the expression of these proteins. Our aim was to investigate whether GBE could attenuate ischemia/reperfusion-induced apoptosis in cardiac myocytes and its potential mechanisms. In the myocardium ischemia reperfusion (IR) rat model, treatment of GBE (400 mg/kg) significantly decreased the cardiomyocyte cell apoptosis and myocardium infarction. Immunohistochemical analysis showed that GBE significantly inhibited I/R-induced increase of myocardial Bax, caspase-3, and cyt-c proteins expression. Western blot analysis confirmed results of immunohistochemical analysis. It is most likely that multiple pathways are involved in IR-induced apoptosis in rat myocardium cells. Therefore, these results demonstrate that GBE exhibits significant protective effect against myocardial I/R injury in rat heart, which is related to down-regulate Bax, cyt-c and caspase-3. Bcl-2 overexpression might prevent IR-induced apoptosis by inhibiting cytochrome c release from the mitochondria and block activation of caspase-3.
Insights
Ginkgo biloba extract (GBE) reduces heart cell death and damage after ischemia/reperfusion injury in rats. GBE achieves this by down-regulating key proteins involved in apoptosis, such as Bax, cytochrome c, and caspase-3.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Pharmacology
Background:
- Myocardial apoptosis, regulated by Bax, cytochrome c, and caspase-3, is crucial in ischemia/reperfusion (IR) injury.
- Ginkgo biloba extract (GBE) is suspected to possess antiapoptotic properties, potentially through protein expression modulation.
Purpose of the Study:
- To investigate the potential of GBE in attenuating IR-induced apoptosis in cardiac myocytes.
- To elucidate the underlying mechanisms of GBE's cardioprotective effects.
Main Methods:
- Establishment of an IR rat model.
- Administration of GBE (400 mg/kg) to assess its effects on cardiomyocyte apoptosis and myocardial infarction.
- Immunohistochemical and Western blot analyses to evaluate the expression levels of Bax, cytochrome c, and caspase-3 proteins.
Main Results:
- GBE treatment significantly reduced cardiomyocyte apoptosis and myocardial infarction in the IR rat model.
- GBE administration inhibited the IR-induced increase in myocardial Bax, caspase-3, and cytochrome c protein expression.
- Western blot analysis corroborated the immunohistochemical findings, confirming GBE's impact on these apoptotic markers.
Conclusions:
- GBE demonstrates a significant protective effect against myocardial IR injury in rats.
- The cardioprotective mechanism of GBE involves the down-regulation of Bax, cytochrome c, and caspase-3 expression.
- These findings suggest GBE's potential therapeutic role in mitigating cardiac damage associated with IR injury.
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