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Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
Published on: October 10, 2011
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Two-color in situ hybridization of whole-mount mouse embryos
Kristin K Biris1, Terry P Yamaguchi
1Frederick National Lab of Cancer Research, NCI, NIH, Frederick, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 10, 2013
Abstract:
RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization that can be used to accurately assess the expression of multiple genes with contrasting or overlapping expression patterns in whole mouse embryos.
