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In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 5, 2026

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Published on: August 25, 2021

Genetic cell ablation.

Damien Grégoire¹, Marie Kmita

¹Laboratory of Genetics and Development, Institut de Recherches Cliniques de Montréal (IRCM), Université de Montréal, Montréal, Québec, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|December 10, 2013

Summary

This summary is machine-generated.

Targeted cell ablation uses genetic tools to precisely remove cells, aiding the study of organ development, tissue repair, and regeneration processes in living organisms.

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Last Updated: May 5, 2026

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Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

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Area of Science:

Developmental Biology
Regenerative Medicine
Cell Biology

Background:

Targeted cell ablation is crucial for understanding in vivo cell functions.
Significant advancements in genetic tools have refined cell ablation control over the past 20 years.

Purpose of the Study:

To provide a comprehensive overview of available genetic tools for targeted cell ablation.
To emphasize the specificity of different genetic ablation methods.

Main Methods:

Review of existing literature on genetic tools for cell ablation.
Analysis of the specificity and applications of various ablation techniques.

Main Results:

Identification and categorization of distinct genetic tools for targeted cell ablation.
Evaluation of the specificity of each tool in different biological contexts.

Conclusions:

Genetic tools offer precise control for studying cell functions in vivo.
Understanding tool specificity is key for effective application in organogenesis, homeostasis, and regeneration research.