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Resonant waveguide grating biosensor-enabled label-free and fluorescence detection of cell adhesion
Natalya Zaytseva1, Jeffery G Lynn, Qi Wu
1Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York 14831, USA.
Sensors and Actuators. B, Chemical
|December 10, 2013
Summary
Cell adhesion mechanisms were studied using biosensors. Receptor signaling, specifically from beta-2 adrenergic receptors (β2-AR), influences cell adhesion to extracellular matrix, impacting drug discovery.
Area of Science:
- Cell Biology
- Biophysics
- Pharmacology
Background:
- Cell adhesion to the extracellular matrix (ECM) is crucial for cellular functions.
- Investigating receptor signaling's impact on cell adhesion remains underexplored.
- Label-free biosensors offer potential for studying cell adhesion dynamics.
Purpose of the Study:
- To develop and utilize label-free and fluorescent biosensor approaches to investigate cell adhesion.
- To examine the influence of receptor signaling on cell adhesion to ECM.
- To explore the effects of beta-2 adrenergic receptor (β2-AR) agonists and antagonists on cell adhesion.
Main Methods:
- Development of resonant waveguide grating biosensors.
- Utilizing label-free and fluorescent detection methods.
- Employing an engineered HEK-293 cell line expressing GFP-tagged β2-AR.
Main Results:
- Cell adhesion is sensitive to temperature and ECM coating.
- Distinct mechanisms govern cell adhesion under varying conditions.
- β2-AR agonists, but not antagonists, enhanced cell adhesion to fibronectin via signaling.
Conclusions:
- The dual biosensor approach effectively investigates cell adhesion mechanisms.
- This method can identify drugs and receptor signaling pathways affecting cell adhesion.
- Understanding β2-AR signaling is key to modulating cell adhesion processes.
