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Updated: May 5, 2026
Preparation of Pooled Human Platelet Lysate pHPL as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
Published on: October 30, 2009
Human platelet lysate as a promising growth-stimulating additive for culturing of stem cells and other cell types
Ya D Shanskii1, N S Sergeeva, I K Sviridova
1P. A. Gertsen Moscow Research Oncological Institute; N. I. Pirogov Russian National Research Medical University; I. M. Sechenov First Moscow State Medical University, Ministry of Health Care of the Russian Federation, Moscow, Russia. prognoz.06@mail.ru.
Abstract:
We compared the composition and biological activity of fetal calf serum and platelet lysate from donor platelet concentrate. In platelet lysate, the concentrations of alkaline phosphatase, lactate dehydrogenase, creatinine, and mineral metabolism parameters were lower, while parameters of lipid and protein metabolism were higher than in fetal calf serum. The concentrations of growth factors (platelet-derived (AA, AB, BB), vascular endothelial, insulin-like, and transforming growth factor β) in platelet lysate 1.7-148.7-fold surpassed the corresponding parameters in fetal calf serum. After replacement of fetal calf serum with platelet lysate in the culture medium (0, 25, 50, 75, and 100%), the count of multipotent mesenchymal stromal cells on day 7 (in comparison with day 1) increased by 154.8, 206.6, 228.2, 367.7, and 396.5%, respectively. Thus, platelet lysate can be an adequate non-xenogenic alternative for fetal calf serum.
