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[A novel SELEX method for screening of HIV-1 P24 antigen]
Shao-Bing Zhan1, Xiao-Guang Zhang, Hong-Xia Li
1National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 10052, China.
Objective:
To screen aptamers that can bind P24 antigen tightly and specificly, and verify its specificity and affinity.
Methods:
Polycarbonate PCR plate was coated with P24 antigen and SELEX technology was used to screen aptamer on the PCR plate. The primary and secondary structure of these aptamers was analyzed by software. Through HRP labeled streptavidin and biotin labeled aptamers, the affinity and specificity of obtained aptamers were verified by ELISA.
Results:
The polycarbonate PCR plate could be coated with P24 antigen. Electrophoretic analysis showed the aptamers had been enriched. Sequence aligment analysis showed that these aptamers have consensus sequence and their apatial structure was multiple; ELISA verified that aptamers had high affinity with P24 antigen.
Conclusion:
A simple method was established for screening aptamers that can bind HIV-1 P24 antigen specificly and tightly.
