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[Construction and characterization of EGFP reporter gene labeled Sindbis virus]
Ling-Ling Deng1, Jiang-Jiao Li, Yan Wei
1College of Life Science & Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Objective:
To construct and characterize EGFP reporter gene labeled Sindbis virus (SINV).
Methods:
The reporter gene EGFP was inserted into the genome of infectious clone pBR-XJ160 by using multi-fusion long fragment PCR method. Then apply reverse genetic manipulation technique to rescue and obtain EGFP labeled SINV.
Results:
We successively obtained labeled SINV, which has good fluorescent expression characteristics and genetic stability.
Conclusion:
The labeled virus can be seen in living cells and living body, and this serves as a good tool for cell and tissue tropism and biological function study of viruses. This study laid a foundation for further studying the cell tropism, biological functions and infection mechanism of SINV.
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