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Related Experiment Videos

Non-organ specific thermostable tissue antigens.

S Kaise, F Milgrom

    Immunological Investigations
    |October 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    New non-organ-specific tissue antigens (NOST) were found in all human tissues. These antigens appear in sera of older individuals and patients with certain diseases, but not in lepromatous leprosy.

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    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Thermostable ethanol insoluble antigens (BE antigens) are present in human tissues but absent in normal serum.
    • Previously described BE antigens were organ-specific.

    Purpose of the Study:

    • To identify and characterize non-organ-specific tissue antigens (NOST).
    • To develop a method for detecting NOST in pathological human sera.

    Main Methods:

    • Antisera were generated against BE preparations of human organs, selected for lack of organ specificity, and absorbed with pooled human serum.
    • Enzyme immunoassay inhibition was used to detect NOST BE antigens.

    Main Results:

    • Non-organ-specific tissue antigens (NOST) were identified in all human tissues and dispersed cells, absent in normal serum.

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  • Positive results (inhibition >35%) were found in 48% of sera from individuals aged 70+, 30% with end-stage renal disease, 18% of renal graft recipients, and 44% with lymphoma/leukemia.
  • No positive results were observed in patients with lepromatous leprosy.

    • Conclusions:

    • NOST are detectable in human sera and their presence is associated with aging and specific pathological conditions.
    • The developed immunoassay is a convenient method for detecting NOST in pathological sera.