Related Experiment Video
Updated: May 5, 2026

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Published on: October 15, 2014
Procedure for microbial identification based on Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass
Gabriel A March Rosselló1, María P Gutiérrez Rodríguez, Raúl Ortiz de Lejarazu Leonardo
1Service of Microbiology, University Clinic Hospital of Valladolid, Valladolid, Spain.
Abstract:
Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry is a widely used proteomic technique in clinical microbiology laboratories, and enables microbial identification directly from clinical samples. This study seeks to establish a protocol for bacterial identification from monomicrobial urine samples that have tested positive in the screening with Sysmex UF-1000i (Sysmex Corporation, Kobe, Japan). Sysmex UF-1000i counts ≥1 × 10(7) bacteria/mL indicate a sufficient bacterial concentration to allow direct identification from urine, with 87.5% sensitivity. Microbial identification from urine with Sysmex UF-1000i counts between 1 × 10(5) and 1 × 10(7) bacteria/ml requires preincubation to obtain the adequate amount of bacteria needed for analysis, and 91.7% sensitivity thus being achieved.
Related Concept Videos
Automated Microbial Diagnostics
MALDI-TOF Mass Spectrometry
Matrix-Assisted Laser Desorption Ionization (MALDI)
Methods of Classification and Identification
Microbiota of the Urogenital Tract
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

